Agrisera/PsbA | D1 protein of PSII, DE-loop/AS10 704/-免疫在线蚂蚁淘旗下平台-

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Agrisera/PsbA | D1 protein of PSII, DE-loop/AS10 704/

来自 : 发布时间：2024-05-04


product information
Background		The psbA gene has been cloned from many species of plants, green algae, and cyanobacteria. The psbA gene is located in the chloroplast genome and encodes for the D1 protein, a core component of Photosystem II. PsbA/D1 is rapidly cycled under illumination in all oxygenic photobionts. Tracking PsbA pools using the Global PsbA antibody can show the functional content of Photosystem II in a wide range of samples. Alternative names: 32 kDa thylakoid membrane protein, photosystem II protein D1
Immunogen		KLH-conjugated synthetic peptide, amino acids 234-242 of D1 protein P83755 (AtCg00020)
Host		Rabbit
Clonality		Polyclonal
Clone
Purity		Serum
Format		Lyophilized
Quantity		50 µl
Reconstitution		For reconstitution add 50 µl of sterile water.
Storage		store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications		Western Blot (WB)
Related products		AS05 084 \| PsbA antibody, C-terminal rabbit antibody AS01 016 \| anti-PsbA C-terminal hen antibody AS06 124A \| anti-PsbA, N-terminal rabbit antibody AS01 016S \| PsbA protein standard for quantitation and positive control Plant and algal protein extraction buffer Secondary antibodies
Additional information		antibody will detect 23 kDa N-terminal fragment

application information
Recommended dilution		1 : 10 000 on thylakoid fraction (WB)
Expected \| apparent MW		38 \| 28-30 kDa
Confirmed reactivity		Arabidopsis thaliana, Chlamydomonas reinhardii, Hordeum vulgare, Medicago truncatula Physcomitrella patens, Pisum sativum, Silene vulgaris, Sinapsis alba, Spinacia oleracea, Synechococcus sp. PCC 7942, Synechocystis sp. PCC6803
Predicted reactivity		dicots including: Cucumis sativus, Glycine max, Nicotiana tabacum, Ricinus communis, Vitis vinifera, monocots including: Oryza sativa, Zea mays, trees: Populus balsamifera
Not reactive in		no confirmed exceptions from predicted reactivity known in the moment
Additional information		antibody is recognizing a 23 kDa fragment in spinach and Arabidopsis thylakoids for usage on total cell extracts the dilution needs to be determined experimentally
Selected references		Kale et al. (2017). Amino acid oxidation of the D1 and D2 proteins by oxygen radicals during photoinhibition of Photosystem II. Proc Natl Acad Sci U S A. 2017 Mar 14;114(11):2988-2993. doi: 10.1073/pnas.1618922114. Mazur et al. (2016). Overlapping toxic effect of long term thallium exposure on white mustard (Sinapis alba L.) photosynthetic activity. BMC Plant Biol. 2016 Sep 2;16(1):191. doi: 10.1186/s12870-016-0883-4. Kowalewska et al. (2016). Three-dimensional visualization of the internal plastid membrane network during runner bean chloroplast biogenesis. Dynamic model of the tubular-lamellar transformation. The Plant Cell March 21, 2016 tpc.01053.2015. Karlsson et al. (2015). The Arabidopsis thylakoid transporter PHT4;1 influences phosphate availability for ATP synthesis and plant growth. Plant J. 2015 Aug 8. doi: 10.1111/tpj.12962. Malnoë et al. (2014). Thylakoid FtsH Protease Contributes to Photosystem II and Cytochrome b6f Remodeling in Chlamydomonas reinhardtii under Stress Conditions. Plant Cell, Jan 21. Sobrino-Plata et al. (2014). Glutathione is a key antioxidant metabolite to cope with mercury and cadmium stress. Plant Soil, DOI 10.1007/s11104-013-2006-4. Block et al. (2013). Functional Modeling Identifies Paralogous Solanesyl Diphosphate Synthases that Assemble the Side Chain of Plastoquinone-9 in Plastids. J Biol Chem. Aug 2.Spetea et al. (1999). GTP bound to chloroplast thylakoid membranes is required for light-induced, multienzyme degradation of the photosystem II D1 protein. PNAS 96: 6547-6552.

application example

$\"western$

Thylakoid membranes from Arabidopsis (0.05-0.75 µg of Chl) were separated on 14%AA+ 6M urea gels and blotted 1h to PVDF. Blots were blocked immediately following transfer in 5% milk solution for 1h at room temperature with agitation. Blots were incubated in the primary antibody at a dilution of 1: 20 000 o.n. at 4°C with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at room temperature with agitation. Blots were incubated in secondary antibody (donkey anti-rabbit IgG horse radish peroxidase conjugated, from GE-Healthcare) diluted to 1:5 000 . The blots were washed as above and developed for 5 min with ECL-Plus detection reagent according to the manufacturers instructions. Exposure time was 1 min in CCD camera Fuji4000.

Courtesy Professor Cornelia Spetea-Wiklund, University of Ghotenburg, Sweden

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发布于： 2024-05-04 阅读（）

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