KLH-conjugated synthetic peptide derived from available plant, algal and cyanobacterial PsbA sequences, including Arabidopsis thaliana UniProt: A4QJR4, TAIR: AtCg00020 , Oryza sativa P0C434, Populus alba Q14FH6, Physcomitrella patens Q6YXN7, Chlamydomonas reinhardtii P07753, Synechocystis sp. P14660 and many others

Store at +4°C for 12-18 months. A preservative may be added for long time storage up to 2 years.

AS01 016S | PsbA protein standard for a quantitative western blotAS05 084PRE | PsbA | D1 protein of PSII, C-terminal , pre-immune serumAS08 084A | anti-PsbA | D1 protein of PSII, C-terminal, rabbit antibodyAS11 1786  | anti-PsbA N-terminal, rabbit antibodyAS10 704 | anti-PsbA | D1 protein of PSII, DE-loop, rabbit antibodyAS13 2669 | anti-PsbA | D1 protein of PSII, phosphorylated, rabbit antibody

Plant and algal protein extraction buffer

Secondary antibodies

Due to biology of PsbA (D1) protein a number of degradation products can apprear in a sample and may be observed when using anti-PsbA antibodies, including products having apparent molecular weights of 24kDa and 16kDa. D1 degradation is a complex set of events and the products observed can be influenced by both the extraction procedure and the physiology of the cells prior to harvest. Third, cross-linking may occur between D1 and cytochrome b559, shifting the protein higher in the gel. In cyanobacteria (PCC7942), three different bands were competed out by preincubating the antibody with the PsbA free peptide, indicating that all bands are indeed PsbA and its precursors or breakdown products. Competition assays were also performed with spinach and Chlamydomonas, confirming the identity of PsbA bands.

Anti-PsbA antibodies will not detect D2 protein, as the peptide used to generate PsbA antibodies has no homology to the D2 sequence.